Queen Beryl is the ruler of the Negaverse. From her Negaverse, Beryl attempts to free the powers of the Negaforce. With these powers, she would become capable of conquering Earth and its universe. During this story, her goals are to obtain energy, to free the powers of the Negaforce, and to obtain the Imperium Silver Crystal, the only weapon capable of foiling Queen Beryl's plans.

Jadeite was Queen Beryl's first general (chronologically in the series). His mission was to gather energy to activate the powers of the Negaverse.

One of Queen Beryl's agents. He poses himself as a big time business man. He prefers to use his powers to amplify the energy of his victims before collecting it. He was destroyed in the episode A Friend in Wolf's Clothing.

Zoisite is one of Queen Beryl's servants. He was trained by Malachite, another servant of Queen Beryl. His relationship between himself and Malachite is much more than just trainer and trainer. These two have developped a semi-romantic relationship. In the storyline, Zoisite is mainly responsible for retrieving the seven rainbow crystals that were scattered on Earth. He has an absolute hatred of Tuxedo Mask, more so than the Sailor Scouts. Eventually, he dies in episode #31 "A Reluctant Princess" for having disobeyed Queen Beryl's orders.

Malachite is one of Queen Beryl's more powerful agents. With his assistant and trainee Zoisite, their mission is to recover the Imperium Silver Crystal, so that Queen Beryl can procede to release the forces of the Negaverse. He has a semi-romantic relationship with Zoisite (the only person he can work with as a team). He has an absolute hatred of the Sailor Scouts (especially Sailor Moon), much more than for Tuxedo Mask. That way, both Zoisite and Malachite complement each others' hatred for the good guys.